The Lineberger Comprehensive Cancer Center's (LCCC) Pre-Clinical Genomic Pathology Lab (gPATH) is a medium throughput facility providing comprehensive genomic services for investigators by employing Next Generation Sequencing (NGS) and/or NanostringTM technology. The mission of the Pre-Clinical Pathology Laboratory is to supports translational human cancer genomics research at the University of North Carolina - Chapel Hill. The gPATH facility performs bio sample processing and analyses for the molecular pathologic and genomic characterization of patient-derived specimens in preclinical research projects and clinical protocols. Our laboratory employs highly-automated, as well as manual, platforms for reproducible medium-throughput nucleic acid extraction, library preparation, and next-generation sequencing of tissue, cells and blood samples. Our goal is to provide a resource that balances rigor and flexibility to meet the needs of academic and clinical research, and that can ultimately produce optimized and validated processes for adoption in clinical labs.