Genome Signature
Human Immune Cell Genome and Transcriptome – Genome Signature
The IMGF focuses on the preparation of Next Generation Sequencing Libraries of immune cells and solid tumor and non-tumor tissues. Libraries are sequenced by the UNC High Throughput Sequencing Facility. The IMGF provides basic bioinformatic analysis of the sequence as an option.
Next Generation Sequencing Library Preparation
| Library Type | Targeted Sequences |
| 10X Genomics single cell Indexed cDNA libraries of mRNAs for RNA transcriptome and the variable (5′) regions of T or B lymphocyte antigen receptors are synthesized from single cell suspensions. Library concentration and quality are determined by fluorometry and gel electrophoresis. Indexed libraries are pooled and submitted to the UNC HTSF for sequencing. |
3′ transcriptome
5′ transcriptome |
| Illumina Nextera Flex Whole Exome Sequencing Library Prep (WES)
Library concentration and quality are determined by fluorometry and gel electrophoresis. Indexed libraries are pooled and submitted to the UNC HTSF for sequencing. |
Nextera Flex Whole Exome Sequence:
Genomic DNA is subjected to transposase-mediated fragmentation and sequencing adapter ligation followed by fragment indexing and amplification. |
| Illumina TruSeq RNA Transcriptome Sequencing Libraries (RNAseq) Library concentration and quality are determined by fluorometry and gel electrophoresis. Indexed libraries are pooled and submitted to the UNC HTSF for sequencing. | mRNA Stranded: Indexed cDNA libraries of known mRNA strand origin are synthesized from fragmented and randomly primed total RNA.
TruSeq RNA Exome (total & FFPE RNA): Indexed cDNA libraries are synthesized from total RNA extracted from formaldehyde-fixed and paraffin-embedded (FFPE) tissue sections. |
| Takara 5′ RACE Indexed cDNA libraries of the variable (5′) regions of T and B lymphocyte antigen receptors are synthesized from total RNA by rapid amplification of cDNA ends (5′-RACE). Library concentration and quality are determined by fluorometry and gel electrophoresis. Indexed libraries are pooled and submitted to the UNC HTSF for sequencing. | T cell receptor 5′ amplicon B cell receptor 5′ amplicon |