Immune Signature
Human Immune Cell Cytometric Immunophenotyping
Immune cell phenotypes are determined by labeling with panels of fluorophore- or nanoparticle-conjugated antibodies and subsequent analysis by fluorescence flow cytometry or time of flight mass cytometry. Panel compositions are subject to change without notice. Representative validated panels are listed below but can be customized by consulting IMGF.
Contact us for inquiries regarding custom panels.
T Lymphocyte Assays
| Phenotype | Phenotypic Antigens |
| T Functional Cells (OMIP-042)
T helper cell subsets are characterized using a panel of fluorophore-conjugated antibodies. |
CCR4, CCR6, CCR7, CCR10, CD4, CD8, CD25, CD38, CD45RA, CD127, CXCR3, CXCR5, HLA-DR |
| T Regulatory Cell (13 fluor w/ TIGIT)
T regulatory cell subsets are characterized using a panel of fluorophore-conjugated antibodies. |
CD4, CD8, CD25, CD39, CD45, CD45RA, CD127, CTLA-4, FoxP3, PD-1, TIGIT, Helios |
| CD4, CD8, CD25, CD28, CD45, CD45RA, CD45RO, CD95, CD127, CD197 (CCR7), FoxP3, PD-1 | |
| Functional T Effector & Regulatory Cells (14 fluor panel) A panel of multicolor fluorophore-conjugated antibody probes to characterize cellular activities of T effector and T regulatory cells. |
CD4, CD8, CD25, CD39, CD69, CD45RA, CD103, CD127, CCR7, PD-1, CTLA4, Fox P3, Helios |
| Tumor Infiltrating T Lymphocyte A panel of multicolor fluorophore-conjugated antibody probes to characteristic T lymphocyte antigens is used to measure the abundance of T lymphocyte cell types that regulate anti-tumor activity within the tumor microenvironment. | CD4, CD8, CD25, CD39, CD45RA, CD127, CCR7, CD69, CD103, CCR4 (or CC8), CTLA-4, FoxP3, PD-1 |
| Tetramer CD8 A panel of multicolor fluorophore-conjugated antibody probes to characteristic T lymphocyte antigens and MHC tetramers are used together to measure the abundance of antigen-specific lymphocytes. | Consult with the IMGF staff to develop the panel of tetramers and T cell markers |
B Lymphocyte Assays
| Phenotype | Phenotypic Antigens |
| B Cell (8 fluor) | |
| B Functional Cell (OMIP-047)
A panel of multicolor fluorophore-conjugated antibody probes to characterize subpopulations of B lymphocytes. |
CD3, CD10, CD14, CD16, CD19, CD21, CD27, CD38, IgD, IL-21R, Ki67 |
| B Regulatory Cell A panel of multicolor fluorophore-conjugated antibody probes to characteristic B lymphocyte antigens measures the abundance of a B lymphocyte subpopulation that regulates T lymphocyte function. | CD1d, CD3, CD10, CD14, CD16, CD19, CD24, CD25, CD27, CD38, CD39, CD73, IgM |
Myeloid Cell Assays
| Phenotype | Phenotypic Antigens |
| Myeloid, Dendritic & Myeloid-derived Suppressor Cell
A panel of multicolor fluorophore-conjugated antibody probes to characteristic myeloid cell antigens measures the abundance of myeloid cell subpopulations that can augment or suppress immune cell function. |
CD11b, CD11c, CD14, CD15, CD16, CD33, CD86, CD123, HLA-DR, (CD3/CD19/CD56) |
Multi-Lineage Assays
| Phenotype | Phenotypic Antigens |
| Whole Blood Immune Cell Profile
A panel of nanoparticle-conjugated antibody probes to characterize lymphocytic and myeloid lineage populations of immune cells in peripheral blood. |
CD3, CD4, CD8, CD11c, CD14, CD16, CD19, CD20, CD25, CD27,CD28, CD38, CD45, CD45RA, CD45RO, CD56, CD57,CD66b, CD123, CD127, CD161, CD294, CCR5, CCR6, CCR7, CXCR3, CXCR5, HLA-DR, IgD, TCTγ/δ |
| T, B & Myeloid Cell General Panel
A panel of multicolor fluorophore-conjugated antibody probes to characteristic leukocyte cell antigens measures the abundance of T, B & myeloid cell subpopulations. |
CD4, CD8, CD25, CD28, CD45RA, CD45RO, CD95, CD127, CD197, (CCR7), FoxP3, PD-1, CD19, CD21, CD24, CD27, CD38, CD45, IgD, IgM, CD86, CD123, CD14, CD33, HLA-DR, CD15, CD16, CD11c, CD11b, (CD3/CD19/CD56) |