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Tumor and Non-Tumor Tissue Processing

Process Description
Isolation of Peripheral Blood Mononuclear Cells (PBMCs) A subset of circulating immune cells, mostly lymphocytes and monocytes, are separated from all other components of whole blood.
Cryopreservation or Thawing Cryopreserved Cell Suspensions Single cell suspensions are cryopreserved as a concentrated suspension which is frozen and stored at -80℃ to preserve viability when thawed and returned to a liquid cell suspension for cellular or molecular analyses.
Tumor & Non-tumor Tissue Dissociation A small sample of a tumor or solid tissue is dis-aggregated into a suspension of single cells to facilitate the analysis of the cellular and soluble mediator composition of the sample.
Enrichment or Depletion of 1-4 Cell Phenotypes Specific subpopulations of cells that express  characteristic cell surface antigens, can be separated from a heterogeneous mixture of cell types by affinity adsorption to magnetic microbeads that are coated with an antibody to the cell surface antigen.
Pleural Fluid Processing The cellular and liquid fractions of a sample of pleural fluid recovered by thoracentesis are separated for analysis.


Nucleic Acid Isolation and Quality Control

Source Description
Whole blood Genomic DNA and/or RNA are isolated from cell suspensions by silica spin-column adsorption chromatography. DNA and RNA concentrations and purity are determined by spectrophotometry, fluorometry and gel electrophoresis.
Buccal swab Genomic DNA is isolated from buccal swabs by silica spin-column adsorption chromatography.
Cell suspension Total RNA isolation & QC from less than 500K cells
Total RNA isolation from greater than 500K cells
Genomic DNA isolation & QC
Genomic DNA & total RNA isolation & QC