Human Tissue Processing
Tumor and Non-Tumor Tissue Processing
| Process | Description |
| Isolation of Peripheral Blood Mononuclear Cells (PBMCs) | A subset of circulating immune cells, mostly lymphocytes and monocytes, are separated from all other components of whole blood. |
| Cryopreservation or Thawing Cryopreserved Cell Suspensions | Single cell suspensions are cryopreserved as a concentrated suspension which is frozen and stored at -80℃ to preserve viability when thawed and returned to a liquid cell suspension for cellular or molecular analyses. |
| Tumor & Non-tumor Tissue Dissociation | A small sample of a tumor or solid tissue is dis-aggregated into a suspension of single cells to facilitate the analysis of the cellular and soluble mediator composition of the sample. |
| Enrichment or Depletion of 1-4 Cell Phenotypes | Specific subpopulations of cells that express characteristic cell surface antigens, can be separated from a heterogeneous mixture of cell types by affinity adsorption to magnetic microbeads that are coated with an antibody to the cell surface antigen. |
| Pleural Fluid Processing | The cellular and liquid fractions of a sample of pleural fluid recovered by thoracentesis are separated for analysis. |
Nucleic Acid Isolation and Quality Control
| Source | Description |
| Whole blood | Genomic DNA and/or RNA are isolated from cell suspensions by silica spin-column adsorption chromatography. DNA and RNA concentrations and purity are determined by spectrophotometry, fluorometry and gel electrophoresis. |
| Buccal swab | Genomic DNA is isolated from buccal swabs by silica spin-column adsorption chromatography. |
| Cell suspension | Total RNA isolation & QC from less than 500K cells Total RNA isolation from greater than 500K cells Genomic DNA isolation & QC Genomic DNA & total RNA isolation & QC |