Antibody Panels

New to mass cytometry?

For members of UNC Lineberger who are interested in trying this technology, we offer limited access to pre-titrated, antibody panels for human and mouse markers. For more information, please email miannone@unc.edu.

Protocols for Cell Staining

• Benzonase wash for thawed frozen cells
• Viability stain
• Surface stain
• Intracellular stain for cytokines and transcription factors (optional)
• Iridium stain

Data Analysis Options

Cytobank

The UNC Mass Cytometry and Cell Omics Core utilizes Cytobank Enterprise to upload data files and share with users.

Cytobank is a cloud-based platform that offers analysis of high-dimensional single-cell data. The platform provides access to analytical tools such as SPADE, FlowSOM and CITRUS as well as visualization tools like UMAP and viSNE.

To learn more about Cytobank flow cytometry software, visit Cytobank’s training videos, webinars and tutorials.

Astrolabe Diagnostics

Astrolabe Diagnostics offers cloud-based bioinformatic analysis of mass cytometry data sets. The platform performs unsupervised data cleaning, debarcoding, clustering, cell subset identification (based on canonical markers), statistical analysis and data visualization.

To learn more, visit Astrolabe’s YouTube channel.

To learn about the core’s price competitive access to Astrolabe’s services, please email miannone@unc.edu.