Histology Resources
The purpose of this page is to provide useful links and instructional material to help PSC clients follow GLP and proper histological techniques.
General Histology and Pathology References.Pathology Outlines is a free pathology resource
Tips for Better Histology is a troubleshooting and best practice guide from Leica Biosystems
Digital Pathology Association is a nonprofit organization dedicated to advancing the field of digital pathology
A Summary of IHC Methodology from ThermoFisher
Overview, Tips and Tricks for IHC-Paraffin Samples from BioRad
Preventing Non-Specific Staining in your IHC from R&D Systems
The Human Protein Atlas is a very helpful resources for checking biomarker expression in different tissues and cells.
Antibody Validation offers specific advice on antibody validation from the Human Protein Atlas.
Fluorescence Microscopy Reference Standards from ThermoFisher
Spectra Viewer from ThermoFisher is a useful tool for comparing excitation / emission spectra for a large number of fluorophores. You can also check spillover between channels if you know the filter specifications.
The GENIE algorithm is used by PSC to develop custom tissue segmentation macros
Leica Microsystems is a manufacturer of histology systems hardware and software utilized in the PSC
Definiens offers Architect and Tissue Studio software used in PSC for image analysis
Visiopharm software is used for image analysis in the PSC