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The purpose of this page is to provide useful links and instructional material to help PSC clients follow GLP and proper histological techniques.

The Human Protein Atlas — https://www.proteinatlas.org/ — This is a very helpful resource for checking biomarker expression in different tissues and cells.

Regarding Antibody Validation — https://www.proteinatlas.org/about/antibody+validation#ihom

A Summary of IHC Methods — https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-biology-learning-center/protein-biology-resource-library/pierce-protein-methods/ihc-immunodetection.html

Fluorescence Microscopy Standards — http://www.thermofisher.com/us/en/home/references/molecular-probes-the-handbook/tools-for-fluorescence-applications-including-reference-standards-and-optical-filters/fluorescence-microscopy-reference-standards-and-antifade-reagents.html

IHC Overview — https://www.bio-rad-antibodies.com/immunohistochemistry-antibodies-tips-and-tricks.html?utm_source=marketo&utm_medium=IHC&utm_campaign=IHC-cancer-16&mkt_tok=eyJpIjoiWmpWbE9UWTJaVFZqTURVeiIsInQiOiI0ZzRQS1pkXC80ZE5MaXVRZXhBMzFsWVlYYSt0eWdvNGZaQmpXSERhMDFsNWJZQ01BeTRaN3JSVmJoTXlXRFlmTWFyblpIditab3AyeFhJcWdSODVqQWNVVTlYMU9EaUpCZmJNdEpvWllFTWs9In0%3D

Preventing Non-Specific Staining — https://www.rndsystems.com/resources/protocols/preventing-non-specific-staining

A Free, Updated Pathology Resource — http://www.pathologyoutlines.com/index.html

About GENIE — http://www.genie.lanl.gov/ — PSC uses the GENIE algorithm to develop custom tissue segmentation macros.

Spectra Viewer — https://www.thermofisher.com/us/en/home/life-science/cell-analysis/labeling-chemistry/fluorescence-spectraviewer.html — This is a useful tool for comparing excitation / emission spectra for a large number of fluorophores. You can also check spillover between channels if you know the filter specifications.

Digital Pathology Association — https://digitalpathologyassociation.org/

Leica Biosystems — https://www.leicabiosystems.com/ — Manufacturer of histology systems (hardware and software).

Definiens — http://www.definiens.com/ — PSC uses Definiens Architect and Tissue Studio for image analysis.

Visiopharm — https://www.visiopharm.com/ — PSC uses Visiopharm software for image analysis.