Animal Histopathology

Animal Histopathology Core

To sup­port UNC’s Line­ber­ger Com­pre­hens­ive Can­cer Cen­ter, the An­im­al His­to­path­o­logy Core Fa­cil­ity, run by a board-cer­ti­fied veter­in­ary patho­lo­gist, of­fers high-qual­ity pre­par­a­tion and pro­cessing of frozen and fixed tis­sue sec­tions, along with edu­ca­tion re­gard­ing ap­pro­pri­ate har­vest­ing, fix­a­tion, Immunology, and se­lec­tion of his­to­lo­gic­al pro­ced­ures.

   

Services

Routine Histology

  • Processing
  • Embedding
  • Sectioning (paraffin and frozen)
  • H&E and special stains
  • Model selection and tissue collection recommendations
  • Consultation with veterinary pathologist by appointment
  • IHC and in situ hybridization by special arrangement

NOTE: Tissues must be submitted in appropriate fixative in clearly labeled containers. Any potential human or animal pathogens, especially in frozen blocks, must be declared. Both human and animal tissues may be submitted.

This core facility is under the direction of Faculty Director Stephanie Montgomery, PhD, DVM, Dipl. ACVP, and Facility Director Dawud Hilliard, MPM, HTL (ASCP) QIHC. Staff includes Lily Wai, Histotechnologist; Traci Raley, Histotechnologist; and Amanda Brown, Histotechnologist.

 

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Our Core Mission

The goal of the LCCC Animal Histopathology Core (AHC) is to provide high quality, affordable histology support to the UNC biomedical research community.  The AHC adds value through a convenient campus location, customizable services, subsidized pricing, and access to board-certified veterinary pathologists.  Major services include tissue embedding and sectioning (frozen and paraffin), routine and special stains, consultation on animal study design, tissue collection, immunohistochemistry (IHC), immunofluorescence (IF), and pathology consultation.  The AHC also provides strong support to other Core Facilities.

Equipment

The AHC is a fully equipped histopathology laboratory occupying over 800 sq. in a central, on-campus location.

The  Animal Histopathology Core equipment includes:

2 Leica Histoembedder Stations

4 Baush & Lomb 4x-100x(oil) Microscopes

3 Leica ATC 200 4x-100x(oil) Microscopes

1 Ken-a-Vision Stereo Microscope

1 Swift Digital Microscope

3 Leica RM2255 Microtomes

1 Leica RM2145 Microtme

4 Boekel Scientific Lighted Tissue Flotation Baths

1 Thermo Scientific Cryostat

1 Bond Max for IHC

1 Ventana Discovery Ultra Automated IHC/ISH Research Slide Staining System

1 Leica Auto StainerXL

1 Leica ASP300S Tissue Processor

1 Leica ASP6025 Tissue Processor

1 Nikon Fluorescent Microscope

 

If you would like to stop by to tour the lab, make use of our microscopes, or have any questions regarding our equipment please feel free to contact us. We would be happy to help.

 

 

Publication

For Publication

We would appreciate that all work provided by the Animal Histopathology Core which is included in a publication record should acknowledge the contribution of the core facility.  Proper acknowledgment of our core facility enables us to obtain financial and other support so we may continue to provide essential services at a low cost.  It would be appreciated if you sent a copy of the accepted manuscript and/or published paper to lcccanimalhistopathology@med.unc.edu.  

In addition, if any facility personnel makes a substantial intellectual and/or experimental contribution to a publication they deserve recognition just as any other co-author.  This is essential for the professional development of our staff.  We recommend scientists follow the “ABRF Recommended Guidelines for Authorship on Manuscripts”.  

Please include in your publication acknowledgements:
Animal histopathology was performed within the LCCC Animal Histopathology Core Facility at the University of North Carolina at Chapel Hill. The LCCC Animal Histopathology Core is supported in part by an NCI Center Core Support Grant (CA16086) to the UNC Lineberger Comprehensive Cancer Center.