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UNC School of Medicine | UNC Lineberger Comprehensive Cancer Center

Nucleic Acid Quality Control

The Translational Genomics Lab (TGL) performs nucleic acid quality control on the following material types. Our quality control protocol uses about 3 µL of sample.

If possible, please send samples with at least 10-15 µL to ensure there is enough sample remaining after quality control to perform downstream genomic assays.

Our Laboratory Management System (LIMS) and freezers are designed to accept individually labeled tubes. If possible, please avoid submitting samples in tube strips or plates.

 

Genomic DNA

  • Caption available.
    Invitrogen Qubit 1X dsDNA Broad Range Assay. Image provided by Thermo Scientific.
    Quantity

    • Instrument: Thermo Scientific Qubit Flex Fluorometer (Cat Q33327)
    • Kit: Invitrogen Qubit 1X dsDNA Broad Range Assay (Cat Q33266)
  • Quality
    • Instrument: Agilent TapeStation 4200 (Cat G2991AA)
    • Kit: Agilent Genomic DNA ScreenTape Analysis (Cat 5067-5365)

 

Cell Free DNA

  • Quantity and Quality
    • Instrument: Agilent TapeStation 4200 (Cat G2991AA)
    • Kit: Agilent Cell-free DNA ScreenTape Analysis (Cat 5067-5630)

 

Total RNA

  • Quantity
    • Instrument: Thermo Scientific Qubit Flex Fluorometer (Cat Q33327)
    • Kit: Invitrogen Qubit RNA Broad Range Assay (Cat Q10211)
      Caption available.
      4200 TapeStation System. Image provided by Thermo Scientific.
  • Quality
    • Instrument: Agilent TapeStation 4200 (Cat G2991AA)
    • Kit: Agilent RNA ScreenTape Analysis (Cat 5067-5576)

 

Cell Free RNA

  • Quantity and Quality
    • Instrument: Agilent TapeStation 4200 (Cat G2991AA)
    • Kit: Agilent High Sensitivity RNA ScreenTape Analysis (Cat 5067-5579)

 

Next-Generation Sequencing Library

  • Quantity
    • Instrument: Thermo Scientific Qubit Flex Fluorometer (Cat Q33327)
    • Kit: Invitrogen Qubit 1X dsDNA High Sensitivity Assay (Cat Q33231)
  • Quality
    • Instrument: Agilent TapeStation 4200 (Cat G2991AA)
    • Kit: Agilent D1000 DNA ScreenTape Analysis (Cat 5067-5582)

 

Next-Generation Sequencing Pool

  • Caption available.
    Qubit Flex Fluorometer. Image provided by Thermo Scientific.
    Quantity

    • Instrument: Thermo Scientific Qubit Flex Fluorometer (Cat Q33327)
    • Kit: Invitrogen Qubit 1X dsDNA High Sensitivity Assay (Cat Q33231)
  • Quality
    • Instrument: Agilent TapeStation 4200 (Cat G2991AA)
    • Kit: Agilent D1000 High Sensitivity ScreenTape Analysis (Cat 5067-5584)