Short-read Sequencing

The Translational Genomics Lab (TGL) performs high-throughput sequencing using the following short-read technologies.

In addition to generating our own libraries, we also accept user submitted libraries for sequencing if they are compatible with Illumina sequencers. When submitting libraries, it is our recommendation for us to perform our own quality control before pooling the libraries.

Our sequencers use patterned flow cells, which are sensitive to even small percentages of adapter dimers (~0.5-1.0%). We look for these during QC and will be able to identify any libraries that might put the entire pool at risk of failure on the sequencer.

Our Laboratory Management System (LIMS) and freezers are designed to accept individually labeled tubes. If possible, please avoid submitting samples in tube strips or PCR plates.

Illumina iSeq 100

• Use: Library quality control (QC) and pool balancing
  

  

• Input Material: Illumina compatible sequencing libraries
• Recommended Library Concentration: >4 nM
• Flow Cells and Kits Offered:
  • i1 (300 cycles): 4 million clusters

Illumina NextSeq 2000

• Use: Medium-throughput sequencing
• Input Material: Illumina compatible sequencing libraries
• Recommended Library Concentration: >4 nM

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  Flow Cells and Kits Offered:
  • P1 (100 cycles): 0.1 billion clusters
  • P1 (300 cycles): 0.1 billion clusters
  • P1 (600 cycles): 0.1 billion clusters
  • P2 (100 cycles): 0.4 billion clusters
  • P2 (200 cycles): 0.4 billion clusters
  • P2 (300 cycles): 0.4 billion clusters
  • P2 (600 cycles): 0.3 billion clusters
  • P3 (100 cycles): 1.2 billion clusters
  • P3 (200 cycles): 1.2 billion clusters
  • P3 (300 cycles): 1.2 billion clusters
  • P4 (50 cycles): 1.8 billion clusters
  • P4 (100 cycles): 1.8 billion clusters
  • P4 (200 cycles): 1.8 billion clusters
  • P4 (300 cycles): 1.8 billion clusters

Illumina NovaSeq X

• Use: High-throughput sequencing
• Input Material: Illumina compatible sequencing libraries
• Recommended Library Concentration: >4 nM
  

  

• Flow Cells and Kits Offered:
  • 1.5B (100 cycles): 1.5 billion clusters
  • 1.5B (200 cycles): 1.5 billion clusters
  • 1.5B (300 cycles): 1.5 billion clusters
  • 10B (100 cycles): 10 billion clusters
  • 10B (200 cycles): 10 billion clusters
  • 10B (300 cycles): 10 billion clusters
  • 25B (100 cycles): 25 billion clusters
  • 25B (200 cycles): 25 billion clusters
  • 25B (300 cycles): 25 billion clusters