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The Tissue Culture Facility (TCF) is UNC Lineberger’s most comprehensive and widely used core facility. The TCF is a supply center and provides UNC Lineberger members and UNC colleagues with a wide variety of products.

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Contact

Amy Patrylick
Phone: (919) 966-4324
Fax: (919) 966-5782

Hours

Monday through Friday, from 9 a.m. – 1 p.m. and 2 p.m. – 5 p.m.The facility is closed for lunch from 1 – 2 p.m.

Location

Room 318, UNC Lineberger Comprehensive Cancer Center

Media, Reagents and Supplies

The TCF stocks media, media supplements, serum, salt solutions, antibiotics, cell culture flasks and dishes, and many other in vitro cell culture and molecular biology-related items.

Centralized Cell Repository

Over 400 cell lines are available, as well as various computerized catalogues (American Type Culture Collection, NIGMS Human Genetic Mutant Cell Repository). Cell lines include normal and transformed mammalian, avian, insect, amphibian/reptile, and hybridoma cells.

Screening and Testing

The TCF tests the stocked Fetal Bovine Serum for quality, efficacy, and usefulness in research. The TCF partners with Genetica Cell Line Services (part of LabCorp) to offer the most stringent qPCR-based Mycoplasma contamination testing for cell or DNA samples.The TCF also utilizes Genetica Cell Line Services for STR profiling of cell lines.

Molecular Biology Support Program

The TCF operates the only centralized molecular biology materials program at UNC. Through the program, researchers can obtain almost any enzyme, reagent, or kit for use in molecular biology related work. The TCF has over 500 products from eight suppliers at its facility in the Lineberger building. All of the products are discounted 15-45 percent.

In addition, any products not in stock can be ordered from these suppliers at discount rates with free shipping. The companies currently under contract with the Tissue Culture Facility are: Qiagen, SigmaAldrich, ThermoFisher Scientific, Invitrogen/Gibco, Promega, New England Biolabs, Roche Biochemicals, Corning Plastics, and Biorad.

Leadership

Amy Patrylick, Tissue Culture Facility Manager