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UNC Lineberger Comprehensive Cancer Center

Nucleic Acid Quality Control

The Translational Genomics Lab (TGL) performs nucleic acid quality control on the following material types. Our quality control protocol uses about 3 µL of sample.

If possible, please send samples with at least 10-15 µL to ensure there is enough sample remaining after quality control to perform downstream genomic assays.

Our Laboratory Management System (LIMS) and freezers are designed to accept individually labeled tubes. If possible, please avoid submitting samples in tube strips or plates.

Caption available.
Invitrogen Qubit 1X dsDNA Broad Range Assay. These assays are highly selective for dsDNA over ssDNA, RNA, protein, and free nucleotides. Image provided by Thermo Scientific.

Genomic DNA

  • Quantity
    • Instrument: Thermo Scientific Qubit Flex Fluorometer (Cat Q33327)
    • Kit: Invitrogen Qubit 1X dsDNA Broad Range Assay (Cat Q33266)
  • Quality
    • Instrument: Agilent TapeStation 4200 (Cat G2991AA)
    • Kit: Agilent Genomic DNA ScreenTape Analysis (Cat 5067-5365)

Cell Free DNA

  • Quantity and Quality
    • Instrument: Agilent TapeStation 4200 (Cat G2991AA)
    • Kit: Agilent D1000 High Sensitivity ScreenTape Analysis (Cat 5067-5584)
Caption available.
The Qubit Flex Fluorometer accurately and quickly measures the concentration of DNA, RNA, or protein in up to eight samples simultaneously. Image provided by Thermo Scientific.

Total RNA

  • Quantity
    • Instrument: Thermo Scientific Qubit Flex Fluorometer (Cat Q33327)
    • Kit: Invitrogen Qubit RNA Broad Range Assay (Cat Q10211)
  • Quality
    • Instrument: Agilent TapeStation 4200 (Cat G2991AA)
    • Kit: Agilent RNA ScreenTape Analysis (Cat 5067-5576)

Cell Free RNA

  • Quantity and Quality
    • Instrument: Agilent TapeStation 4200 (Cat G2991AA)
    • Kit: Agilent High Sensitivity RNA ScreenTape Analysis (Cat 5067-5579)

Next-Generation Sequencing Library

Caption available.
The 4200 TapeStation system is an established, high-throughput automated electrophoresis platform for nucleic acid sample quality control. Image provided by Thermo Scientific.
  • Quantity
    • Instrument: Thermo Scientific Qubit Flex Fluorometer (Cat Q33327)
    • Kit: Invitrogen Qubit 1X dsDNA High Sensitivity Assay (Cat Q33231)
  • Quality
    • Instrument: Agilent TapeStation 4200 (Cat G2991AA)
    • Kit: Agilent D1000 DNA ScreenTape Analysis (Cat 5067-5582)

Next-Generation Sequencing Pool

  • Quantity
    • Instrument: Thermo Scientific Qubit Flex Fluorometer (Cat Q33327)
    • Kit: Invitrogen Qubit 1X dsDNA High Sensitivity Assay (Cat Q33231)
  • Quality
    • Instrument: Agilent TapeStation 4200 (Cat G2991AA)
    • Kit: Agilent D1000 High Sensitivity ScreenTape Analysis (Cat 5067-5584)

 

To learn more about TGL, email lccctgl@unc.edu or come by 5118A Marsico Hall.

TGL collaborates with the Office of Genomics Research for project management. To submit a consultation request, email ogr@unc.edu